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pbmn mcherry parkin  (Addgene inc)


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    Structured Review

    Addgene inc pbmn mcherry parkin
    Pbmn Mcherry Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+parkin/pBMN-mCherry-Parkin+(Plasmid+%2359419)/pmc12759083-20-0-2
    Average 93 stars, based on 13 article reviews
    pbmn mcherry parkin - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: ER stress and mitochondrial dynamics: a tale of a wandering phosphatase DUSP28
    Article Snippet: YFP-Drp1 (WT), YFP-Drp S637A and YFP-Drp1 S637D were obtained as a gift from Prof. Luca Scorrano, Universita degli Studi di Padova, Italy ( ). mCherry-Drp1 (49152), pMD2.G (12259), psPAX2 (12260), mCherry-Parkin (23956), DsRed2-Mito-7 (55838), pmRFP-LC3 (21075) and FLAG-Stx17 (45911) were obtained from Addgene.

    Construct:

    Article Title: ANKZF1 helps to eliminate the stress-damaged mitochondria by LC3-mediated mitophagy
    Article Snippet: .. Constructs used in this study include the following: EBFP2-N1 (Addgene, # 54595), mCherry Parkin (Addgene), mCherry-Mito-7 (Addgene #55102), ptfLC3 (Addgene #21074), Mito GFP, RFP-LC3, pHAGE-mt-mKeima (Addgene #131626). .. ANKZF1-FL-GFP, Δ210-ANKZF1-GFP, Δ330-ANKZF1-GFP, and Δ370-ANKZF1-GFP constructs were generated by using standard restriction digestion-based molecular cloning technique, and desired genes were ligated with digested pEGFPN1 (clonetech) empty vector backbone.

    Article Title: ANKZF1 helps to eliminate stress-damaged mitochondria by LC3-mediated mitophagy
    Article Snippet: .. Constructs used in this study include the following: EBFP2-N1 (Addgene, # 54595), mCherry Parkin (Addgene), mCherry-Mito-7 (Addgene #55102), ptfLC3 (Addgene #21074), Mito GFP, RFP-LC3, pHAGE-mt-mKeima (Addgene #131626). .. ANKZF1-FL-GFP, Δ210-ANKZF1-GFP, Δ330-ANKZF1-GFP, Δ370-ANKZF1-GFP, Δ410-ANKZF1-GFP, Δ450-ANKZF1-GFP constructs were generated by using standard restriction digestion-based molecular cloning technique, and desired genes were ligated with the digested pEGFPN1 (Clontech) empty vector backbone.

    Article Title: ANKZF1 helps to eliminate stress-damaged mitochondria by LC3-mediated mitophagy
    Article Snippet: .. The EGFP-Parkin construct was generated by subcloning of Parkin from mCherry-Parkin (Addgene #23956) into the EGFP-N1 plasmid backbone. ..

    Article Title: The N-terminal Segment of the Human ANKZF1 negatively regulates its internal mitochondrial targeting signal to prevent its mitochondrial localization
    Article Snippet: .. Plasmid constructs used in this study include the following: pCMV0-OCT-HA-eGFP (Addgene, #67479), pCMV0-SMAC-HA-eGFP (Addgene #67486), both the plasmids were a gift from Richard Kahn ( ). mCherry-Parkin (Addgene #23956) was a gift from Richard Youle , and Mito BFP (Addgene, #55248) ( ) was a gift from Michael Davidson. .. Δ73-ANKZF1-GFP, Δ210-ANKZF1-GFP, Δ220-ANKZF1-GFP, Δ230-ANKZF1-GFP, Δ240-ANKZF1-GFP, Δ250-ANKZF1-GFP, Δ330-ANKZF1-GFP, Δ370-ANKZF1-GFP, and Δ410-ANKZF1-GFP constructs were generated in the lab by using standard restriction digestion-based molecular cloning technique, and desired genes were digested with XhoI and KpnI restriction enzymes (NEB) and were ligated to pEGFP-N1 (Clontech) empty vector backbone.

    Clone Assay:

    Article Title: LMNA R644C Associates With Hepatic Steatosis in a Large Cohort and Increases Cellular Lipid Droplet Accumulation in Vitro
    Article Snippet: .. The LMNA open reading frame was cloned into mCherry-Parkin, Addgene plasmid #23956 (replacing the Parkin open reading frame), via the BspEI and BamHI sites. mCherry-Parkin was a gift from Richard Youle (Addgene plasmid #23956; http://n2t.net/addgene:23956; RRID:Addgene 23956)15. .. The R644C variant of LMNA was generated using the QuikChange II site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) and verified via Sanger sequencing.

    Plasmid Preparation:

    Article Title: LMNA R644C Associates With Hepatic Steatosis in a Large Cohort and Increases Cellular Lipid Droplet Accumulation in Vitro
    Article Snippet: .. The LMNA open reading frame was cloned into mCherry-Parkin, Addgene plasmid #23956 (replacing the Parkin open reading frame), via the BspEI and BamHI sites. mCherry-Parkin was a gift from Richard Youle (Addgene plasmid #23956; http://n2t.net/addgene:23956; RRID:Addgene 23956)15. .. The R644C variant of LMNA was generated using the QuikChange II site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) and verified via Sanger sequencing.

    Article Title: HTLV-1 Tax induces PINK1-Parkin-dependent mitophagy to mitigate activation of the cGAS-STING pathway
    Article Snippet: The pCMV4-Tax, pCMV4-Tax M22 and VSV-G plasmids were provided by Dr. Shao-Cong Sun. pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid # 131626; http://n2t.net/addgene:131626 ; RRID:Addgene_131626). .. CFP-Parkin was a gift from Richard Youle (Addgene plasmid #47560; http://n2t.net/addgene:47560 ; RRID; Addgene_47560). mCherry-Parkin was a gift from Richard Youle (Addgene plasmid #23956; http://n2t.net/addgene:23956 ; RRID:Addgene_23956). .. HA-p62 was a gift from Qing Zhong (Addgene plasmid #28027; http://n2t.net/addgene:28027 ; RRID:Addgene_28027). pHAGE-eGFP-NDP52 was a gift from Wade Harper (Addgene plasmid#175749; http://n2t.net/addgene:175749 ; RRID:Addgene_175749). pHAGE-FLAG-APEX2-TAX1BP1 was a gift from Wade Harper (Addgene plasmid #175761; http://n2t.net/addgene:175761 ; RRID:Addgene_175761). psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260).

    Article Title: ANKZF1 helps to eliminate stress-damaged mitochondria by LC3-mediated mitophagy
    Article Snippet: .. The EGFP-Parkin construct was generated by subcloning of Parkin from mCherry-Parkin (Addgene #23956) into the EGFP-N1 plasmid backbone. ..

    Article Title: The N-terminal Segment of the Human ANKZF1 negatively regulates its internal mitochondrial targeting signal to prevent its mitochondrial localization
    Article Snippet: .. Plasmid constructs used in this study include the following: pCMV0-OCT-HA-eGFP (Addgene, #67479), pCMV0-SMAC-HA-eGFP (Addgene #67486), both the plasmids were a gift from Richard Kahn ( ). mCherry-Parkin (Addgene #23956) was a gift from Richard Youle , and Mito BFP (Addgene, #55248) ( ) was a gift from Michael Davidson. .. Δ73-ANKZF1-GFP, Δ210-ANKZF1-GFP, Δ220-ANKZF1-GFP, Δ230-ANKZF1-GFP, Δ240-ANKZF1-GFP, Δ250-ANKZF1-GFP, Δ330-ANKZF1-GFP, Δ370-ANKZF1-GFP, and Δ410-ANKZF1-GFP constructs were generated in the lab by using standard restriction digestion-based molecular cloning technique, and desired genes were digested with XhoI and KpnI restriction enzymes (NEB) and were ligated to pEGFP-N1 (Clontech) empty vector backbone.

    Generated:

    Article Title: ANKZF1 helps to eliminate stress-damaged mitochondria by LC3-mediated mitophagy
    Article Snippet: .. The EGFP-Parkin construct was generated by subcloning of Parkin from mCherry-Parkin (Addgene #23956) into the EGFP-N1 plasmid backbone. ..

    Subcloning:

    Article Title: ANKZF1 helps to eliminate stress-damaged mitochondria by LC3-mediated mitophagy
    Article Snippet: .. The EGFP-Parkin construct was generated by subcloning of Parkin from mCherry-Parkin (Addgene #23956) into the EGFP-N1 plasmid backbone. ..



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